RIDA®GENE Color Compensation Kit II is intended for generating a Color Compensation File for duplex and triplex real-time PCR experiments on the LightCycler® 1.5 and 2.0. The generated Color Compensation File can be applied to analyze real-time multiplex PCR experiments of RIDA®GENE real-time PCR kits on the LightCycler® 1.5 and 2.0.
In a real-time multiplex PCR, the wavelengths of light emitted by the reporter dyes may overlap, causing one channel to pick up signals (crosstalk) from a dye measured by another channel. This crosstalk of fluorescence signal can result in incorrect data unless a correction is made by using a Color Compensation File. Color compensation is used to subtract fluorescence crosstalk from a reporter dye into inappropriate channels outside of its dominant emission channel.
|Test format||3 reactions|
|Incubation time||5 min|