For in vitro diagnostic use. The RIDA®LINE Parvovirus B19 tests are line blots for the detection and identification of IgG or IgM antibodies against the parvovirus B19 in human serum or plasma. The tests should be used for confirmation of positive results or clarification of unspecific findings with other serological methods. Due to the separately coated recombinant components of the virus protein and the possibility of IgG avidity testing, this test procedure allows a statement about the presence of antibodies with different specificity and therefore a better interpretation of the different stages of the infection.
The avidity determination of Parvovirus B19 IgG can be done with the RIDA® Aviditätsreagenz (Art. No. LB0023).
IgM antibodies can be detected approximately 10 days after the infection. That is usually at the beginning of the symptoms. After 30 – 60 days, IgM antibodies are mostly no longer detectable. IgG antibodies can already be found some days after the increase of IgM.
The Parvovirus consists of two structural proteins VP1 and VP2. VP1 is subdivided for the blot into two segments (VP-N and VP-C), which allows a better interpretation of the immune response at an early stage. IgG antibodies are formed usually against the linear epitopes of the C-terminal end (VP-C) after first infection. These antibodies are significantly reduced within 6 – 12 months or disappear altogether. Thus, an increased IgG reactivity against the VP-C indicates a fresh or just recently obtained infection. The linear epitopes at the N-terminal end (VP-N) usually remain for life, but may also decrease in some people. However, antibodies formed against structural epitopes of VP2 are detectable for life-time. Therefore, the line blot uses a complete VP2 protein in addition to VP-N. IgG antibodies against the NS-1 can be involved in the interpretation of some problems and be indicative for a probably persistent Parvovirus B19 infection.
The cleaned (free of fusion proteins), recombinant antigens were applied to a nitro-cellulose membrane. Following that, the membrane was cut into individual blot strips. The strip is incubated with the sample (serum or plasma) that is to be examined. During incubation, the specific antibodies present in the sample will bind to the corresponding protein bands. After removal of unbound antibodies by a washing step, a second incubation with anti-human IgG conjugate (peroxidase) and a subsequent color reaction, specifically bound antibodies against the parvovirus B19 antigens become visible as blue bands on the strips.
Please also pay attention to our ELISA for antibody detection against Parvovirus B19 (Article no.: K6021 and K6031)
|Test format||Article no. LB6023|
Parvovirus B19 IgG (20 determinations)
Article no. LB6033
Parvovirus B19 IgM (20 determinations)
The test kit contains 20 blot strips coated with recombinant Parvovirus antigens, synthesized in prokaryotic and eukaryotic cells.
|Incubation time||60 min / 45 min / 5-10 min at room temperature|