Intended use:

For in vitro diagnostic use. The RIDA®QUICK Verotoxin / O157 Combi test is a immunochromatographic rapid test for the qualitative determination of Verotoxins (syn. Shiga toxins) and of Escherichia coli serotype O157 in enrichment cultures.

General information:

Enterobacteriaceae of the species E. coli are a normal part of the intestinal flora of humans as well as of many farm animals. E. coli can be viewed as facultative pathogenic through different, often plasmid-coded pathogenicity factors or pathogenicity factors transferred through phages. This includes, in particular, enterohemorrhagic E. coli (EHEC), which was identified in 1977 and is the primary cause of severe illnesses such as hemorrhagic colitis (HC) and hemolytic uremic syndrome (HUS). They are capable of producing two cytotoxins (Verotoxin 1 and 2). A multitude of other serotypes that can cause severe illnesses besides the O157 prototype have been described since then. Therefore, every STEC should be considered to be a potential EHEC.

The current recommended approach in the diagnostic testing of STEC/EHEC is overnight incubation in mTSB broth containing Mitomycin C as an inductor for the formation of shigatoxins with the subsequent immunological detection of the shigatoxins as well as the shigatoxingens and the isolation of the pathogene from the enrichment broth.

The RIDA®QUICK Verotoxin / O157 Combi rapid assay enables the extremely specific and simultaneous detection of shigatoxins and the highly virulent Serotype O157 within 20 minutes from the supernatant of the enrichment broth (e.g. RIDA® Anreicherungsbouillon ; Z1003 ; 25 tubes).

Highlights:

  • Antigen detection from an enrichment culture using a fast assay
  • Unique assay combination for the simultaneous detection of verotoxins and/or E. coli O157
  • An innovative technology enables the color-differentiated detection of both pathogens
  • Sensitive detection of all humanpathogenic strains of Shiga toxin/Verotoxin-producing organisms
  • Specific capturing of all E. coli O157 strains (sorbitol-fermenting and non-sorbitol-fermenting)

Accessories:

Specifications
Art. No. N2203
Test format 20 cassettes
Incubation time The test result can be read off after 15 minutes.
Detection limit Stx 1: 2 ng/ml
Stx 2: 0.5 ng/ml
Sensitivity for Stx 1 / Stx 2: 85.0 %
for O157: 90.9 %
Specificity for Stx 1 / Stx 2: 98.7 %
for O157: 98.5
Additional information Positive controls are available as separate accessories for the corresponding RIDA®QUICK rapid assays.
Files
Instructions
SDS
Similar products
RIDA®GENE Viral Stool Panel III
RIDA®GENE Viral Stool Panel...
For in vitro diagnostic use. RIDA®GENE Viral Stool Panel III is a multiplex real-time RT-PCR for the direct, qualitative detection and differentiat...
RIDASCREEN® Helicobacter
RIDASCREEN® Helicobacter
For in vitro diagnostic use. RIDASCREEN® Helicobacter is an enzyme immunoassay (EIA) for the qualitative detection of Helicobacter pylori-specific ...
RIDA®QUICK Helicobacter
RIDA®QUICK Helicobacter
For in vitro diagnostic use. RIDA®QUICK Helicobacter is an immunochromatographic rapid test (ICT) for the qualitative detection of Helicobacter pyl...

Start typing and press Enter to search

ridascreen-cryptosporidium-giardia-combi-easy-kit