For in vitro diagnostic use. The RIDASCREEN® Bordetella PT tests are enzyme immunoassays for the quantitative determination of IgA or IgG antibodies against Bordetella pertussis toxin in human serum. The tests should be used for confirmation purposes when there is a suspected case of infection with bordetella pertussis or for clarifying the immune status.
Bordetella pertussis is the causative agent of whooping cough. The incubation period takes 7 – 21 days. Specific antibodies are normally detectable with the beginning of the clinical symptoms. Despite an infection or vaccination during childhood, an immunity for life is not generated. Adults with first or second time infection do normally not show the severe picture of whooping cough. However, they represent a source of infection for others. With the ELISA technology, a sensitive method for antibody detection is available with the possibility to differentiate between the individual immunoglobulin classes. This is especially important for the differentiation between a natural infection and an antibody titer due to a vaccination. Specific IgG and IgM are increased following infection or vaccination; whereas, specific IgA is increased only following infection.
Common antigens most frequently used for ELISA are pertussis toxin (PT) and filamentous haemagglutinin (FHA) which are also included in vaccines. Anti-PT antibodies are specific for B. pertussis whereas anti-FHA are less specific due to cross-reactivity with other microbial antigens (e.g. other Bordetella species, Mycoplasma pneumonia, E.coli, Haemophilus species). For this reason in routine diagnosis only the measurement of anti-PT antibodies is recommended.
Pertussis toxin is coated to a microwell plate. Antibodies in the patient samples bind to the antigens and are determined during the second incubation step by using enzyme-labelled anti-human antibodies (the conjugate). The enzyme converts the colourless substrate (H2 O2 /TMB) to a blue end product. The enzyme reaction is stopped by adding sulphuric acid and the colour of the mixture switches from blue to yellow at the same time. The final measurement is carried out at 450 nm on a photometer using a reference wavelength 620 nm.
Please also pay attention to our ELISA (Article no. K2511, K2521 and K2531) for antibody detection against Bordetella IgA, IgG and IgM.
|Test format||Article no. K2611|
Bordetella PT IgA (96 determinations)
Article no. K2621
Bordetella PT IgG (96 determinations)
Microtiter plate with 96 wells (12 strips with 8 wells each; strips can be divided into single wells)
|Incubation time||3 x 30 min at 37 ºC|