Intended use:

For in-vitro diagnostics. The RIDASCREEN® Chikungunya Virus tests are enzyme immunoassays for the qualitative detection of IgG or IgM antibodies to the Chikungunya virus in human serum or plasma.

The tests are intended for use in cases of suspected Chikungunya virus infection.

General Information:

Chikungunya viruses are enveloped, single-strand RNA viruses of the genus Alphavirus in the Togaviridae family. They have a diameter of approximately 60 nm.

There are five different variants of the virus, based on geographic distribution: West African, Central African, East/South African, Indian Ocean and Asian.

Chikungunya viruses are transmitted to humans mainly by mosquitoes of the Aedes aegypti or Aedes albopictus species.

In humans, the virus causes Chikungunya fever. After a 1 – 6 day incubation period, the illness begins with the abrupt onset of joint pain, frequently with biphasic fever, myalgia and nausea. The fever generally lasts just a few days. Other symptoms frequently develop as the illness progresses, including swollen lymph nodes, maculopapular exanthema, headache and exhaustion. The illness normally abates after approximately two weeks, but joint pain can persist for months. Hemorrhagic complications can develop in very rare cases. Lasting damage and death are rare. Alphavirus infections are uncommon in Europe, but imported or travel cases can arise.

As a result of the immune system’s response, the body produces specific antibodies to the pathogen after being infected with the Chikungunya virus. These antibodies can be detected in the serum or plasma with the help of immunological methods. The selection of the pathogen-specific antigen used and the applied test method are important for the validity of a test.

Test principle:

Anti-human IgG or IgM antibodies are bound on a microtiter plate. IgG or IgM antibodies present in patient specimens bind to the anti-human IgG or IgM antibodies. Unbound specimen material is removed by washing. The Chikungunya virus antigens are then deposited on the plate. A wash step follows and then biotinylated Chikungunya virus antibodies are added. After another wash step, enzyme-marked Streptavidin is pipetted, which binds to the immobilized Chikungunya-specific immune complexes. The enzyme changes a colorless substrate (H2O2/TMB) into a blue end product. The enzymatic reaction is stopped by the addition of sulfuric acid. This addition causes the color to change from blue to yellow. The final measurement is taken in a photometer at 450 nm (reference wavelength  ≥ 620 nm).


Art. No. K81**
Test format Microtiter plate with 96 wells (12 strips with 8 wells each; strips can be divided into single wells)

Article no. K8121
Chikungunya Virus IgG capture (96 determinations)

Article no. K8131
Chikungunya Virus IgM µ-capture (96 determinations)
Incubation time 60 min at 37°C
3x 30 min at room temperature (20-25 °C)
15 min at room temperature (20-25 °C)
Sensitivity 98.7 % (IgG)
100 % (IgM)
Specificity 100 % (IgG)
100 % (IgM)
Spanish (German) (German) (English) (English) (French) (French) (Italian) (Italian) (Spanish) (Spanish)
Similar Products
For in vitro diagnostic use. RIDASCREEN® Helicobacter is an enzyme immunoassay (EIA) for the qualitative detection of Helicobacter pylori-specific ...
RIDASCREEN® Zika Virus IgG capture, IgM µ-capture
RIDASCREEN® Zika Virus IgG ...
For in vitro diagnostic use. The RIDASCREEN® Zika Virus tests are enzyme immunoassays for the qualitative detection of IgG or IgM antibodies to the...
RIDASCREEN® Malaria Ab-Screening
RIDASCREEN® Malaria Ab-Scre...
For in vitro diagnostic use. The RIDASCREEN® Malaria Ab-Screening test is an enzyme immunoassay for the qualitative detection of IgG and IgM antibo...

Start typing and press Enter to search