For in vitro diagnostic use. The RIDASCREEN® EBV VCA test is an enzyme immunoassay for the quantitative determination of IgG or IgM antibodies against VCA from the Epstein Barr Virus (EBV) in human serum.
The test should be used for confirmation purposes when there is a suspected case of EBV infection or for clarifying the immune status.
After infection with EBV, specific antibodies are formed against the pathogen as a result of the response from the immune system. By using immunological methods, it is possible to determine the antibodies in the serum. The test method used and the choice of the pathogen-specific antigen both have a significant influence on the test result. Because it is possible to differentiate between the different immunoglobulin classes and to determine the antibodies against different virus proteins, more precise statements about the immunological status of a patient can be made in the enzyme immunoassay.
Purified antigens are coated to a microwell plate. Antibodies in the patient samples bind to the antigens and are determined during the second incubation step by using enzyme-labelled anti-human antibodies (the conjugate). The enzyme converts the colourless substrate (H2O2/TMB) to a blue end product. The enzyme reaction is stopped by adding sulphuric acid and the colour of the mixture switches from blue to yellow at the same time. The final measurement is carried out at 450 nm on a photometer using a reference wavelength ≥ 620 nm.
|Test format||Article no. K6721|
EBV VCA IgG (96 determinations)
Article no. K6731
EBV VCA IgM (96 determinations)
Microtiter plate with 96 wells (12 strips with 8 removable wells each)
|Incubation time||3 x 30 min at 37 °C|