For in vitro diagnostic use. The RIDASCREEN® HSV 1+2 tests are enzyme immunoassay for the quantitative determination of IgG or IgM antibodies against Herpes simplex virus type 1 and 2 (HSV 1 and HSV 2) in human serum.
The tests are based on total antigen and reacts with antibodies against both types in parallel. It should be used for screening for IgG antibodies against HSV.
After infection with HSV, specific antibodies are formed against the pathogen as a result of the response from the immune system. By using immunological methods, it is possible to determine the antibodies in the serum. The test method used and the choice of the pathogen-specific antigen both have a significant influence on the test result.
Purified antigens are coated to a microwell plate. Antibodies in the patient samples bind to the antigens and are determined during the second incubation step by using enzyme-labelled anti-human antibodies (the conjugate). The enzyme converts the colourless substrate (H2O2/TMB) to a blue end product. The enzyme reaction is stopped by adding sulphuric acid and the colour of the mixture switches from blue to yellow at the same time. The final measurement is carried out at 450 nm on a photometer using a reference wavelength ≥ 620 nm.
|Test format||Article no. K5021|
HSV 1+2 IgG (96 determinations)
Article no. K5031
HSV 1+2 IgM (96 determinations)
Microtiter plate with 96 wells (12 strips with 8 wells each; strips can be divided into single wells)
|Incubation time||3 x 30 min at 37 °C|