For in vitro diagnostic use. The RIDASCREEN® Taenia solium IgG test is an enzyme immunoassay for the qualitative determination of IgG antibodies against Taenia solium and its cysticerci (bladder worms) in human serum. The test should be used for confirmation purposes when there is a suspected case of infection with Taenia solium.
Cysticercosis is a disease characterized by the harboring of the larval form (cysticercus) of Taenia solium in any tissue or organ. Although many sites of infection have been documented, the larvae are often found associated with the central nervous system. Presence of the cysticerci in the brain may cause increased cranial pressure, seizures and altered mental states. The disease is acquired by ingestion of T. solium eggs out of which the larvae slip in the intestine. This may happen from a number of different routes such as food contaminated with feces or contaminated water. Additionally, humans can be infected with the tapeworm itself by uptake of larvae containing meat. If eggs are set free in such tapeworm carriers, an endogenous infection with cysticercus will happen. Diagnosis of cysticercosis usually requires multiple methods such as radiography and serology. Although use of specific antigens has helped to increase ELISA’s sensitivity and specificity, significant cross reactions with other diseases (such as echinococcosis) is still a problem. All positive serology samples should be confirmed by an other test method such as the western blot.
Purified antigens are coated to a microwell plate. Antibodies in the patient samples bind to the antigens and are determined during the second step by using enzyme-labelled Protein A (the conjugate). The enzyme converts the colourless substrate (H2O2/TMB) to a blue end product. The enzyme reaction is stopped by adding sulphuric acid and the colour of the mixture switches from blue to yellow at the same time. The final measurement is carried out at 450 nm on a photometer using a reference wavelength ≥ 620 nm.
|Test format||Article no. K7721|
Taenia solium IgG (96 determinations)
Microtiter plate with 96 wells (12 strips with 8 wells each; strips can be divided into single wells)
|Incubation time||3 x 15 min at room temperature|