The RIDASCREEN® Tetanus IgG test is a quantitative enzyme immunoassay for specific detection of IgG antibodies against the tetanus toxoid in human serum. The test should be used for determination of the immune status before or after vaccination.
Tetanus (lockjaw) is caused by a toxin produced by Clostridium tetani. Clostridium tetani is an ubiquitous bacterium in soils. After open wounds have become infected, the pathogen penetrates into the tissue where it multiplies anaerobically excreting the toxin. Because immunization is carried out in infancy using a toxoid vaccine, the risk of infection is low in industrialized countries. But, vaccination has to be repeated every 5 – 10 years. Injured people with vaccine protection should receive a booster as a precaution if their last vaccination is more than five years ago. Human antitoxin must be administered when the vaccination status is not clear. The vaccination status can be determined using a modern ELISA. The comparability of measured values is ensured by using a toxoid antigen and matching to international units. Nevertheless, it has to be considered for EIA result validation that both the extent of the immune reaction after an vaccination and the period of vaccine protection may vary from patient to patient.
Purified antigens are coated to a microwell plate. Antibodies in the patient samples bind to the antigens and are determined during the second incubation step by using enzyme-labelled anti-human antibodies (the conjugate). The enzyme converts the colourless substrate (H2O2/TMB) to a blue end product. The enzyme reaction is stopped by adding sulphuric acid and the colour of the mixture switches from blue to yellow at the same time. The final measurement is carried out at 450 nm on a photometer using a reference wavelength ≥ 620 nm.
|Test format||Article no. K3721|
Tetanus IgG (96 Bestimmungen)
Microtiter plate with 96 wells (12 strips with 8 wells each; strips can be divided into single wells)
|Incubation time||3 x 30 min at 37 ºC|