RIDA®GENE Color Compensation Kit IV is intended for generating a Color Compensation File for 5-plex real-time PCR experiments on the LightCycler ® 480II. The generated Color Compensation File can be applied to analyze 5-plex real-time PCR experiments of RIDA®GENE real-time PCR kits on the LightCycler® 480II.
In a 5-plex real-time PCR, the wavelengths of light emitted by the reporter dyes may overlap, causing one channel to pick up signals (crosstalk) from a dye measured by another channel. This crosstalk of fluorescence signal can result in incorrect data unless a correction is made by using a Color Compensation File. Color compensation is used to subtract fluorescence crosstalk from a reporter dye into inappropriate channels outside of its dominant emission channel.
|Test format||3 reactions|
|Incubation time||15 min|