The RIDA®QUICK Rota/Adeno/Noro Combi is a single step immunochromatographic lateral flow assay for the differential qualitative detection of Rotavirus, Adenovirus and Norovirus of Genogroups I and II in human stool specimen.
Rotavirus is a double stranded RNA virus belonging to the Reoviridae family. They are viruses with a low infective dose and their transmission mechanism is direct contact by one person to another by a faecal-oral route and, less frequently, through contaminated water and food. The Rotavirus is one of the main aetiological agents of acute gastroenteritis in the whole world and main causal agent of severe dehydration in children between 6 months and 2 years, both in developing countries, where it shows a high mortality, as well as in developed countries. At the age of 5 years, the majority of children (> 95%) have suffered at least one episode of gastroenteritis caused by Rotavirus. Although the development of vaccines is helping to reduce the incidence, only some countries have managed to implement them in their national immunisation program. Rotavirus is classified into seven antigenic serogroups (A to G). Only Groups A, B and C infect humans, with Group A being the causing factor of almost all cases, both in developed countries and in developing countries.
Adenovirus is the third leading cause of viral gastroenteritis in children (10 – 15 %). They can also cause respiratory diseases and depending on the serotype, diarrhoea, conjunctivitis, cystitis, and others. At least 51 adenovirus serotypes have been identified and in all of them the hexon antigen is present. Mainly Serotypes 40 and 41 are associated with gastroenteritis. The main clinical symptom of gastroenteritis caused by adenovirus is diarrhoea, for 9 to 12 days, also occurring with fever and vomiting.
Norovirus is a type of single-stranded, positive-sense RNA virus belonging to the Caliciviridae family. They are highly contagious and their main transmission routes are by person to person contact and by contaminated food / water. The virus usually causes large epidemics in closed communities (hospitals, homes for the elderly, schools, nurseries, restaurants, cruise ships, etc.), where once it has been introduced, infection propagates very rapidly. Several studies demonstrate that Norovirus is the main cause of viral gastroenteritis at any age worldwide and responsible for almost 50% of gastroenteritis outbreaks. Norovirus are grouped in five genogroups (GGI to GGV). The majority of clinical cases are as a result of strains of the genogroups I and II. In general, GI infections are less frequent than GII infections. The virus is classified into genotypes within each genogroup. Up to 19 different genotypes within genogroup II have been decribed. Of them, GII.4 is the most common, representing close to 60 – 80% of the cases worldwide. They are followed by GII.6, GII.1 and GII.3.
|Test format||kit with 20 single pouched double cassettes and 20 sample diluent bottles|
|Incubation time||15 minutes|
Norovirus GI 87.5%
Norovirus GII 95.0%
Norovirus GI 98.9%
Norovirus GII 96.6%