RIDA®GENE Color Compensation Kit I is intended for generating a Color Compensation File for real-time multiplex PCR experiments on the LightCycler® 480. The generated Color Compensation File can be applied to analyze multiplex real-time PCR experiments of RIDA®GENE real-time multiplex PCR kits on the LightCycler® 480.
In a multiplex real-time PCR, the wavelengths of light emitted by the reporter dyes may overlap, causing one channel to pick up signals (crosstalk) from a dye measured by another channel. This crosstalk of fluorescence signal can result in incorrect data unless a correction is made by using a Color Compensation File. Color compensation is used to subtract fluorescence crosstalk from a reporter dye into inappropriate channels outside of its dominant emission channel.
|Test format||3 reactions|
|Incubation time||15 min|