For in vitro diagnostic use. RIDA®GENE Flu is a multiplex real-time RT-PCR for the direct, qualitative detection and differentiation of influenza viruses (Influenza A, Influenza B and H1N1v) from human nasal and throat swabs. RIDA®GENE Flu real-time RT-PCR is intended for use as an aid in the diagnosis of influenza infection.
The influenza, also called flu, is the most common respiratory infectious disease which is caused by influenza viruses.
Worldwide, 3 – 5 million people are infected with influenza and approximately 250,000 – 500,000 die from this disease each year. The annual influenza epidemics cause serious public health and economic problems.
In the U.S., influenza causes more than 200,000 hospitalizations and more than 36,000 deaths annually. In the season 2010/2011 more than 41,000 cases of influenza were reported in Germany.
Influenza viruses are RNA viruses belonging to the family of Orthomyxoviridae. They are divided into the subtypes A, B and C. Characteristic for influenza viruses is their high mutational variation (antigenic drift) of the surface antigens, hemagglutinin (HA) and neuraminidase (NA). Influenza types A and B cause the annually occuring influenza epidemics whereas infection with influenza C viruses cause milder disease.
Epidemiologically, influenza A viruses play the major role due to diversity: they are responsible for three pandemics in the 20th Century and for the majority of influenza epidemics. The majority of influenza A infections in humans are caused by the H1N1 and H3N2 subtypes. In addition to the antigenic drift induced by mutation, new influenza A subtypes can be generated by reassortment of a human and nonhuman influenza A strain (antigenic shift), which may trigger a pandemic. The influenza A subtype H1N1, is associated with the past and potentially new influenza pandemics (e.g. the Spanish Flu in 1918/19; Swine Flu 2009). Today, this influenza A subtype is known as H1N1v. The transmission of influenza viruses occurs by droplets and aerosols. The incubation period is 1 – 4 days. Clinical symptoms are mainly severe diseases of the respiratory tract with high fever and cough. The onset of symptoms is characteristically abrupt (sudden onset). During severe courses of disease pneumonia and bacterial super-infection can occur, which can be fatal, especially for the elderly and children.
|Test format||real-time RT-PCR with 100 reactions|
|Shelf life||24 months after production|
|Sensitivity||Analytical Sensitivity : ≥ 50 RNA copies per reaction|