Intended use:

For in vitro diagnostic use. RIDA®GENE RSV & hMPV is a multiplex real-time RT-PCR for the direct, qualitative detection and differentiation of respiratory syncytial virus (A/B) and human metapneumovirus (1-4) from human nasal/throat swabs, nasopharyngeal swabs, throat washes and BAL. RIDA®GENE RSV & hMPV real-time RT-PCR is intended for use as an aid in the diagnosis of RSV & hMPV infections.

General Information:

Respiratory syncitial viruses (RSV) belong to the family of Paramyxoviridae and are enveloped single-stranded RNA viruses. There are two groups of RSV, group A and group B, whereas RSV A is most dominant in most years.

RSV is spread worldwide and can lead to an infection of the upper and lower respiratory tract at every age. RSV is transmitted via smear or airborne transfection and patients show symptoms of rhinitis, cold, cough, acute bronchitis and also inflammation of the middle ear. An acute presentation may occur during the presence of a bacterial super infection and, worldwide, around 600 000 people die yearly from either direct or indirect infection with RSV.2 Infants and small children often show an acute presentation which requires hospitalization. Here, symptoms such as fever, cold and tachypnoe occur. 50 – 70 % of infants and small children have a RSV infection in at the age of one, whereas almost 100 % had a RSV infection after the age of two.

Another new representative of Paramyxoviridae is the human metapneumovirus (hMPV). The virus was first isolated in the Netherlands in 2001 and is genetically related to RSV. However, the course of this infection is less severe compared to RSV infections. In many cases infants are affected by this, whereas 15% of the annual cases of bronchitis are also caused by hMPV. Infants, elderly and immunosuppressed patients with severe affections of hMPV usually need hospitalization. Regarding the route of transmission little is known yet. The worldwide occurrence of hMPV is comparable with the seasonal occurrence of influenza in winter.

Specifications
Art. No. PG5905
Test format real-time RT-PCR with 100 reactions
Incubation time Time to result:
RNA Extraction: 45 min
real-time RT-PCR: 90 min
Cross reactivity

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Shelf life 24 months after production
Sensitivity Analytical Sensitivity : ≥ 50 RNA copies per reaction
Analytical reactivity

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Files
InstructionsEnglish
German
SDSPG5905_german.zip (German)
PG5905_english.zip (English)
PG5905_french.zip (French)
PG5905_italian.zip (Italian)
PG5905_spanish.zip (Spanish)
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