For in vitro diagnostic use. RIDA®GENE Gut Balance is a multiplex real-time PCR for the direct qualitative and quantitative detection of Bacteroides– and Cluster XIVa DNA from human stool samples.
90% of the normal human gut flora is populated by two phylogenetic groups which exist in a symbiotic balance. Bacteroides are anaerobic, gram-negative bacteria which are part of the normal gut flora of the intestinal tract. In the large intestine, approximately 1011 Bacteroides/g stool exist and are therefore the dominant bacteria in terms of numbers. The second phylogenetic group is the firmicutes. Clostridium Cluster XIVa are a class of firmicutes to which, besides others, Eubacterium spp. and roseburia spp. belong.
Different sources associate a disbalance of the composition of the gut flora (dysbiosis) with obesity. Here, a decreased number of Bacteroides corresponds to presence of obesity whereas at the same time an increasing number of Eubacterium rectale was detected in patients with obesity.
|Standard range||real-time PCR with 100 reactions|
|Shelf life||24 month after production|
|Sensitivity||Analytical Sensitivity : ≥ 10 DNA copies per reaction|